中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (32): 5793-5798.doi: 10.3969/j.issn.2095-4344.2013.32.008

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

脂肪干细胞与骨髓间充质干细胞成软骨能力的比较

安荣泽,赵俊延,王兆杰   

  1. 遵义医学院第五附属(珠海)医院骨科,广东省珠海市  519100
  • 收稿日期:2012-11-01 修回日期:2012-12-17 出版日期:2013-08-06 发布日期:2013-08-06
  • 通讯作者: 王兆杰,博士,主任医师,教授,硕士研究生导师,遵义医学院第五附属(珠海)医院骨科,广东省珠海市 519100 zhaojiewang@163.com
  • 作者简介:安荣泽,男,1955年生,贵州省威宁县人,彝族,1978年遵义医学院毕业,主任医师,教授,硕士生导师,主要从事骨与关节损伤的修复及重建方面的研究。 yishuihantangshi@163.com
  • 基金资助:

    贵州省科技厅“十五”攻关课题资助项目(黔科通NY字[2006]62-2);珠海市医学重点专科建设基金资助项目(珠卫[2008]80)。

Chondrogenic potential of adipose-derived stem cells versus bone marrow mesenchymal stem cells

An Rong-ze, Zhao Jun-yan, Wang Zhao-jie   

  1. Department of Orthopedics, the Fifth Affiliated (Zhuhai) Hospital of Zunyi Medical College, Zhuhai  519100, Guangdong Province, China
  • Received:2012-11-01 Revised:2012-12-17 Online:2013-08-06 Published:2013-08-06
  • Contact: Wang Zhao-jie, M.D., Chief physician, Professor, Master’s supervisor, Department of Orthopedics, the Fifth Affiliated (Zhuhai) Hospital of Zunyi Medical College, Zhuhai 519100, Guangdong Province, China zhaojiewang@163.com
  • About author:An Rong-ze, Chief physician, Professor, Master’s supervisor, Department of Orthopedics, the Fifth Affiliated (Zhuhai) Hospital of Zunyi Medical College, Zhuhai 519100, Guangdong Province, China yishuihantangshi@163.com
  • Supported by:

    “Tenth Five-year” Key Project of Guizhou Science and Technology Department, No. QianketongNY[2006]62-2*; Construction Fund Project of Zhuhai Key Medical Specialties, No. Zhuwei[2008]80*

摘要:

背景:脂肪干细胞与骨髓间充质干细胞是软骨组织工程中应用较多细胞,二者在生物学特性上有诸多相似之处。
目的:比较脂肪来源和骨髓来源的2种间充质干细胞的成软骨分化能力。
方法:选取3月龄新西兰大白兔,取腹部脂肪,分离提取脂肪干细胞。取兔双侧股骨,采用贴壁筛选法分离提取骨髓间充质干细胞。绘制第3代脂肪干细胞和骨髓间充质干细胞的生长曲线,比较2种细胞的倍增时间。对第3代脂肪干细胞和骨髓间充质干细胞进行成软骨诱导,分别对诱导14 d的2种细胞行甲苯胺蓝染色和Ⅱ型胶原免疫组化染色。
结果与结论:骨髓间充质干细胞原代细胞呈聚集样生长,而脂肪干细胞原代细胞呈单个、散在生长。脂肪干细胞增殖速度要快于骨髓间充质干细胞,倍增时间短于骨髓间充质干细胞h。2种细胞成软骨诱导14 d后,均表达糖胺聚糖和Ⅱ型胶原,且骨髓间充质干细胞成软骨诱导后表达Ⅱ型胶原水平高于脂肪干细胞。说明脂肪干细胞与骨髓间充质干细胞体外增殖皆迅速且稳定,但是脂肪干细胞的生长增殖速度更快。单层培养时,特定条件下,脂肪干细胞与骨髓间充质干细胞均能向软骨细胞转化,但是骨髓间充质干细胞比脂肪干细胞具有更高的潜能。

关键词: 干细胞, 脂肪干细胞, 骨髓间充质干细胞, 骨髓, 脂肪组织, 成软骨分化, Ⅱ型胶原, 间充质干细胞, 省级基金, 干细胞图片文章

Abstract:

BACKGROUND: Adipose-derived stem cells and bone marrow mesenchymal stem cells are used widely in cartilage tissue engineering, and there are many similarities in biological characteristics between two kinds of cells.
OBJECTIVE: To compare the chondrogenic potential of bone marrow mesenchymal stem cells and adipose-derived stem cells in vitro.
METHODS: Adipose-derived stem cells were isolated from the 3-month-old New Zealand white rabbits’ abdomen. Bilateral femurs of rabbits were obtained, and then the bone marrow mesenchymal stem cells were separated with the adherence screening method. The growth curve of the passage 3 adipose-derived stem cells and bone marrow mesenchymal stem cells were drawn, and the doubling time of two kinds of cells was compared. Then the passage 3 adipose-derived stem cells and bone marrow mesenchymal stem cells were treated with chondrogenic induction. After induced for 14 days, the adipose-derived stem cells and bone marrow mesenchymal stem cells were treated with toluidine blue staining and type Ⅱ immunohistochemistry staining respectively. 
RESULTS AND CONCLUSION: Primary bone marrow mesenchymal stem cells showed aggregative growth, while the primary adipose-derived stem cells were in single and scattered growth. The proliferation speed of adipose-derived stem cells was faster than that of bone marrow mesenchymal stem cells, while the doubling time of adipose-derived stem cells was shorter than that of the bone marrow mesenchymal stem cells. After chondrogenic induction for 14 days, both adipose-derived stem cells and bone marrow mesenchymal stem cells could express glycosaminoglycans and type Ⅱ collagen, and the expression level of type Ⅱ collagen in bone marrow mesenchymal stem cells after chondrogenic induction was higher than that in the adipose-derived stem cells. The in vitro proliferation of adipose-derived stem cells and bone marrow mesenchymal stem cells was rapid and stable, but the proliferative ability of adipose-derived stem cells was faster than that of bone marrow mesenchymal stem cells. When cultured in single layer, both adipose-derived stem cells and bone marrow mesenchymal stem cells could transform into chondrocytes under certain conditions, but bone marrow mesenchymal stem cells seemed to be more potential than adipose-derived stem cells.

Key words: stem cells, adipose-derived stem cells, bone marrow mesenchymal stem cells, bone marrow, adipose tissue, chondrogenic differentiation, type Ⅱ collagen, mesenchymal stem cells, provincial grant-supported paper, stem cell photographs-containing paper

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